Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker

Journal: BioMed Research International

Article Title: Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression

doi: 10.1155/2015/159015

Figure Lengend Snippet: The influence of subchronic arsenic exposure on the expression levels of 5-HT 1A receptor, PKA, TrkB, and p-Akt in the cerebral PFC. Eight weeks after exposure with 10 mg/L arsenic or drinking water alone in normal mice and in reserpine pretreated mice, the expression levels of 5-HT 1A receptor and PKA proteins (a) and the expression levels of TrkB, p-Akt (b) in the cerebral PFC of the tested mice were detected by western blot analysis. These protein expression levels were quantitated by microcomputer image device (MCID) image analysis. The lower panel shows the individual protein/ β -actin ratios while the β -actin levels were evaluated as a loading control. Each value/error bar is expressed as the mean ± SD of 8 mice. ∗ P < 0.05, ∗∗ P < 0.01, significant difference compared with normal control group (drinking water alone). # P < 0.05, significant difference from the reserpine group (Res).

Article Snippet: Rabbit polyclonal antibodies against TrkB, CREB, and PKA were purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Aging (Albany NY)

Article Title: The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors

doi: 10.18632/aging.102713

Figure Lengend Snippet: Effects of RSV and suramin on the expression of synapse makers, p-CREB, GFAP as well as Iba-1, in mice carrying the APP/PS1 double mutation. ( A ) the level of SYP protein; ( B ) SNAP-25; ( C ) p-CREB; ( D ) GFAP and ( E ) Iba-1. The values presented are means ± SD. * P <0.05 compared with the WT group; # P <0.05 compared with the APP/PS1 group, as determined by analysis of variance (ANOVA), followed by the Tukey HSD test. Representative western blots are shown beneath D and F . Representative western blots are shown beneath each bar graph.

Article Snippet: RSV, Suramin, U0126 and the SIRT1 Assay Kit (Sigma-Aldrich, USA); methyllycaconitine (MLA) (Tocris Bioscience, UK); TRIzol reagent (Invitrogen, China), SYBR Green PCR master mix (Applied Biosystems, USA); ECL Plus reagent (Merck Millipore, Germany); the near-infrared amyloid-β fluorescent CRANAD-58 probe (ab146926, Abcam Inc., USA); rabbit monoclonal anti-p-Erk1/2 and rabbit monoclonal anti-Erk1/2 antibodies (4370 and 4695, Cell Signaling Technology (CST) Inc., USA); rabbit monoclonal anti-SIRT1 antibody and anti-SYP (synaptophysin) (ab12193 and ab8049; Abcam Inc., USA); rabbit polyclonal anti-α7 nAChR and mouse monoclonal anti-β-actin antibodies (sc-58607 and sc-376421, Santa Cruz Inc., USA); rabbit polyclonal anti SNAP-25 (synaptosomal-associated protein 25), rabbit polyclonal anti CREB, rabbit polyclonal anti CREB (phosphpo-Ser133), rabbit polyclonal anti GFAP and rabbit polyclonal anti-Iba-1 (ionized calcium binding adaptor molecule 1) antibodies (GTX113839, GTX112846, GTX130379, GTX108711 and GTX100042, Gentex Inc., USA) and horseradish peroxidase-conjugated secondary antibody (7076s and 7074s, CST Inc., USA) were obtained from the sources indicated.

Techniques: Expressing, Mutagenesis, Western Blot